African Green Monkey CD4 T Cells
Purified African Green Monkey CD4+ T Cells (Chlorocebus sabaeus) isolated by immunomagnetic selection. High purity for T cell activation assays, immunotherapy research, and checkpoint inhibitor studies.
African Green Monkey CD4 T Cells Overview
African Green Monkey CD4+ T Cells are primary helper T lymphocytes isolated from African green monkey (Chlorocebus sabaeus) peripheral blood. CD4+ T cells coordinate adaptive immune responses and are central to vaccine development and HIV/SIV research.
Common Applications
- Vaccine immunogenicity and T cell recall assays
- SIV/HIV pathogenesis and reservoir studies
- T helper cell polarization (Th1/Th2/Th17)
- Immune checkpoint and co-stimulation research
Product Details
Cells are enriched by positive or negative immunomagnetic selection and cryopreserved. Each lot ships with a Certificate of Analysis documenting viability, purity, and donor information. Available in multiple cell count options.
About the African Green Monkey
Chlorocebus sabaeus
African green monkeys are increasingly important in biomedical research, particularly for infectious disease studies.
Natural hosts for SIV without disease progression, they provide unique insights into viral pathogenesis and represent a valuable model for respiratory virus research.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Origin Lineages at a Glance
Primary Research Areas
T Cell Activation Assays
Measure T cell proliferation, activation markers, and cytokine production.
Immunomodulator Screening
Evaluate effects of immunomodulatory compounds on T cell function.
Checkpoint Inhibitor Research
Study PD-1, CTLA-4, and other checkpoint pathways in relevant species.
CAR-T Development
Source material for chimeric antigen receptor T cell engineering.
Regulatory T Cell Studies
Investigate Treg biology and immunosuppressive mechanisms.
Antigen-Specific Responses
Measure T cell responses to specific antigens and peptides.
| Species | African Green Monkey (Chlorocebus sabaeus) |
|---|---|
| Cell Type | CD4+ T Cells |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | PBMC isolation by Ficoll density gradient, followed by immunomagnetic positive selection (anti-CD4) |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Caribbean Origin ★ MOST COMMON
island
St. Kitts-derived populations with well-documented lineages. Excellent for controlled studies.
African Origin
mainland
Wild-caught or first-generation captive from African source populations.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Typical purity is >90% CD3+ T cells as assessed by flow cytometry. Sub-populations (CD4+, CD8+) are further enriched by immunomagnetic positive selection, with purity typically >85-95% depending on the specific marker and species. A Certificate of Analysis with purity data is included with each lot.
Many anti-human CD antibodies cross-react with african green and rhesus monkey T cell markers, including CD3, CD4, CD8, and CD28. Cross-reactivity varies by clone and species. We can provide guidance on validated antibody clones for your species of interest.
Thaw rapidly in a 37°C water bath until a small ice crystal remains. Transfer dropwise into pre-warmed complete culture medium. Centrifuge at 300g for 10 minutes, aspirate supernatant, and resuspend in fresh medium. Allow 2-4 hours of rest in culture before functional assays for optimal viability and activation response.
Post-thaw viability is typically ≥70% for cryopreserved T cells. Viability is assessed by trypan blue exclusion or flow cytometry (7-AAD/Annexin V). Fresh cells are shipped with viability ≥90%. Recovery and function can be improved with overnight resting before stimulation.
Yes. We support donor reservation, repeat-donor programs, and matched cell sets from the same donor. Donor demographics (age, sex, weight, health status) are available. Contact us with your requirements for custom donor matching.