African Green Monkey Splenocytes
African Green Monkey primary cells (Chlorocebus sabaeus) isolated using validated methods. Available fresh or cryopreserved with viability guarantee. Contact us for cell-specific specifications.
African Green Monkey Splenocytes Overview
African Green Monkey Splenocytes are primary immune cells isolated from African green monkey (Chlorocebus sabaeus) spleen tissue. Splenocytes comprise a mixed immune population including T cells, B cells, NK cells, monocytes, and dendritic cells.
Common Applications
- ELISpot and FluoroSpot assays for antigen-specific responses
- Vaccine immunogenicity testing
- Mixed lymphocyte reactions (MLR)
- Immune cell phenotyping and functional profiling
Product Details
Splenocytes are prepared by mechanical dissociation and red blood cell lysis, then cryopreserved. Each lot ships with a Certificate of Analysis documenting viability, cell count, and donor information. Available in 10M, 25M, and 50M cell counts.
About the African Green Monkey
Chlorocebus sabaeus
African green monkeys are increasingly important in biomedical research, particularly for infectious disease studies.
Natural hosts for SIV without disease progression, they provide unique insights into viral pathogenesis and represent a valuable model for respiratory virus research.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Origin Lineages at a Glance
Primary Research Areas
Immunotoxicology Studies
Assess potential immunotoxic effects of drug candidates on immune cell populations and function.
Vaccine Development
Evaluate immunogenicity and T cell responses to vaccine antigens in preclinical models.
CAR-T & Cell Therapy Research
Source material for developing and testing chimeric antigen receptor constructs and adoptive cell therapies.
Cytokine Release Assays
Screen biologics for potential cytokine release syndrome risk using species-relevant immune cells.
Flow Cytometry & Immunophenotyping
Characterize immune cell subset distributions and surface marker expression profiles.
ADCC & CDC Assays
Evaluate antibody-dependent and complement-dependent cytotoxicity of therapeutic antibodies.
| Species | African Green Monkey (Chlorocebus sabaeus) |
|---|---|
| Cell Type | Splenocytes (Total Spleen Mononuclear Cells) |
| Cell Count | Variable; typically 50-500 × 10⁶ cells per spleen depending on species |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | Mechanical dissociation of spleen tissue through cell strainer, followed by red blood cell lysis |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Caribbean Origin ★ MOST COMMON
island
St. Kitts-derived populations with well-documented lineages. Excellent for controlled studies.
African Origin
mainland
Wild-caught or first-generation captive from African source populations.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Splenocytes contain a mixed immune cell population including T cells (40-60%), B cells (30-50%), NK cells (5-10%), monocytes/macrophages, and dendritic cells. The exact composition varies by species and individual donor. Flow cytometry immunophenotyping data is available on request.
Splenocytes are isolated by gentle mechanical dissociation of the spleen through a cell strainer, followed by red blood cell lysis (ACK buffer). The resulting single-cell suspension is washed and resuspended in culture medium or cryopreservation medium.
Thaw rapidly at 37°C, dilute dropwise into pre-warmed RPMI + 10% FBS. Centrifuge at 300g for 10 minutes. Resuspend in fresh medium and rest overnight before functional assays for optimal recovery.
Post-thaw viability is typically ≥70%. Splenocyte function (proliferation, cytokine production) recovers well after overnight resting. Fresh splenocytes are shipped with ≥90% viability.
Yes. Donor demographics (age, sex, weight, health status) are available. Matched sets (splenocytes + PBMCs + serum from the same donor) can be arranged.