Beagle Peripheral Blood Mononuclear Cells (PBMC)
High-quality Beagle PBMCs (Canis lupus familiaris) isolated by density gradient centrifugation. Available fresh or cryopreserved with guaranteed viability. Ideal for immunology, vaccine development, and cell-based assays in preclinical research.
Beagle Overview
The beagle is the standard canine model for pharmaceutical safety and toxicology, prized for its uniform size, docile temperament, and well-established historical data. It is a required non-rodent species in many regulatory programs.
Quick Facts
Beagle Peripheral Blood Mononuclear Cells (PBMC) are primary immune cells isolated from purpose-bred beagle dog peripheral blood. Canine PBMCs are essential for preclinical safety and immunotoxicology studies, veterinary vaccine development, and comparative immunology.
Common Applications
- Preclinical immunotoxicology and safety pharmacology
- Veterinary vaccine immunogenicity testing
- Canine-specific immune cell phenotyping
- Comparative immunology and cross-species bridging studies
Product Details
PBMCs are isolated from whole blood by density gradient centrifugation. Available fresh (never frozen) or cryopreserved. Each lot ships with a Certificate of Analysis documenting viability, cell count, and donor information.
Immunotoxicology Studies
Assess potential immunotoxic effects of drug candidates on immune cell populations and function.
Vaccine Development
Evaluate immunogenicity and T cell responses to vaccine antigens in preclinical models.
CAR-T & Cell Therapy Research
Source material for developing and testing chimeric antigen receptor constructs and adoptive cell therapies.
Cytokine Release Assays
Screen biologics for potential cytokine release syndrome risk using species-relevant immune cells.
Flow Cytometry & Immunophenotyping
Characterize immune cell subset distributions and surface marker expression profiles.
ADCC & CDC Assays
Evaluate antibody-dependent and complement-dependent cytotoxicity of therapeutic antibodies.
| Species | Beagle (Canis lupus familiaris) |
|---|---|
| Cell Type | Peripheral Blood Mononuclear Cells (PBMCs) |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | Density gradient centrifugation (Ficoll) |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Beagle PBMCs contain lymphocytes (T cells, B cells, NK cells) and monocytes. Typical composition includes 60-80% T cells (CD3+), 5-15% B cells (CD20+), 5-15% NK cells (CD56+), and 5-20% monocytes (CD14+). Exact percentages vary by individual donor and species.
Many anti-human antibodies cross-react with beagle cells due to epitope conservation. We recommend consulting cross-reactivity databases and validating antibodies empirically. Common cross-reactive markers include CD3, CD4, CD8, CD14, CD20, and CD56.
Thaw vials rapidly in a 37°C water bath until a small ice crystal remains. Dilute slowly into pre-warmed complete medium, centrifuge gently (300 × g, 10 min), and resuspend in fresh medium. Rest cells for 1-2 hours before functional assays.
Post-thaw viability is guaranteed ≥70%. Actual viability typically ranges 75-90% when following recommended thawing protocols. Functional recovery may require 2-4 hours of resting.
Yes, we can provide cells from specific donors for longitudinal studies or matched sample requests. Contact us with your requirements for donor availability and additional donor information.