Cynomolgus Monkey CD14+ Monocytes
Purified Cynomolgus Monkey CD14+ monocytes (Macaca fascicularis) for macrophage differentiation, dendritic cell generation, and inflammation research. High purity isolation by immunomagnetic selection.
The Cynomolgus Monkey
Macaca fascicularis
The cynomolgus macaque is the most widely used non-human primate in biomedical research, valued for its close physiological and immunological similarity to humans.
With over 60% genetic homology for key immune receptors, cynomolgus monkeys provide highly translatable data for drug development, making them ideal for immunology, infectious disease, and toxicology studies.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Geographic Origins
Common Research Applications
Cynomolgus Monkey CD14+ Monocytes are research-grade cynomolgus macaque primary cells intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information including viability and purity data.
CD14+ monocytes are innate immune cells that differentiate into macrophages and dendritic cells, supporting inflammation and phagocytosis research.
Common applications
- Monocyte-to-macrophage differentiation
- Dendritic cell generation
- Inflammation research
- Phagocytosis assays
- Innate immunity studies
Collection, processing & handling
Samples are collected and processed using standardized protocols appropriate to the sample type. Packaging and shipping are configured for temperature-controlled logistics to preserve sample integrity for your specific workflow requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Ordering notes
For specific donor criteria, source, volumes, or custom handling requirements, request a quote and include your study specifications. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Macrophage Differentiation
Generate monocyte-derived macrophages for functional studies.
Dendritic Cell Generation
Differentiate monocytes into dendritic cells for antigen presentation studies.
Inflammation Research
Study monocyte activation and inflammatory cytokine production.
Phagocytosis Assays
Evaluate phagocytic function and clearance mechanisms.
| Species | Cynomolgus Monkey (Macaca fascicularis) |
|---|---|
| Cell Type | CD14+ Monocytes |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | PBMC isolation by Ficoll density gradient, followed by immunomagnetic positive selection (anti-CD14) |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Chinese Origin ★ MOST COMMON
mainland
Chinese-origin cynomolgus are no longer exported by China. Cynomolgus macaques are not native to China and were historically imported from Cambodia, Vietnam, Laos, and Indonesia. Current samples are sourced from US breeding colonies established from China-line imports.
Mauritian Origin ★ DEFINED GENETICS
island
Mauritian cynomolgus have limited MHC diversity due to founder effects, making them valuable for immunology studies requiring genetic consistency.
Cambodian Origin ★ SOUTHEAST ASIAN
mainland
Cambodian-origin animals offer a Southeast Asian source with genetics that have not been mixed with other country lineages in the same way seen in some China-line populations.
Vietnamese Origin ★ SOUTHEAST ASIAN
mainland
Vietnamese cynomolgus provide another Southeast Asian option for programs that require distinct lineage sourcing or supply chain diversification.
Indonesian Origin ★ ISLAND ORIGIN
island
Indonesian-origin cynomolgus were among the earliest origins used in preclinical research and represented one of the largest research populations before China entered the market.
Philippine Origin ★ ISLAND ORIGIN
island
Philippine-origin cynomolgus were one of the first origins used broadly in research, with a long history as a trusted and widely used model.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Typical purity is >90% CD14+ monocytes as assessed by flow cytometry. Monocytes are isolated by immunomagnetic positive selection from PBMCs. Purity and viability data are included on the Certificate of Analysis.
Yes. CD14+ monocytes can be differentiated into macrophages using M-CSF (5-7 days) or into monocyte-derived dendritic cells (moDCs) using GM-CSF + IL-4 (5-7 days). Differentiation protocols are available upon request.
Thaw rapidly at 37°C, transfer dropwise into pre-warmed RPMI + 10% FBS. Centrifuge at 300g for 10 minutes. Resuspend in fresh medium and plate immediately. Monocytes are adherent; allow 2-4 hours for attachment before washing non-adherent cells.
Post-thaw viability is typically ≥70%. Monocyte adhesion and phagocytic function recover well after thawing. Fresh cells are shipped with ≥90% viability. For differentiation assays, fresh monocytes are recommended.
Yes. Donor reservation and repeat-donor programs are available. Donor demographics, health status, and HLA typing (if available) can be provided on request.