Cynomolgus Monkey CD16 NK Cells
Purified Cynomolgusmolgus Monkey NK cells (Macaca fascicularis) for cytotoxicity assays, ADCC studies, and NK cell therapy development. Isolated by immunomagnetic selection with high purity.
Cynomolgus Monkey CD16 NK Cells Overview
Cynomolgus Monkey CD16+ NK Cells are primary natural killer cells isolated from cynomolgus macaque (Macaca fascicularis) peripheral blood, selected for CD16 expression. CD16 (FcγRIII) is the Fc receptor responsible for antibody-dependent cellular cytotoxicity (ADCC), making CD16+ NK cells essential for evaluating therapeutic antibody efficacy.
Common Applications
- ADCC assays for therapeutic antibody development
- Fc receptor engagement and effector function studies
- Cancer immunotherapy target validation
- NK cell biology and innate immune profiling
Product Details
Cells are isolated by immunomagnetic selection for CD16+ and cryopreserved. Each lot ships with a Certificate of Analysis documenting viability, purity, and donor information including geographic origin. Available by origin: Cambodian, Chinese, Indonesian, Mauritian, Philippines, Vietnamese, and US Born.
About the Cynomolgus Monkey
Macaca fascicularis
The cynomolgus macaque is the most widely used non-human primate in biomedical research, valued for its close physiological and immunological similarity to humans.
With over 60% genetic homology for key immune receptors, cynomolgus monkeys provide highly translatable data for drug development, making them ideal for immunology, infectious disease, and toxicology studies.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Origin Lineages at a Glance
Primary Research Areas
Cytotoxicity Assays
Evaluate NK cell-mediated killing of target cells.
ADCC Studies
Assess antibody-dependent cellular cytotoxicity for therapeutic antibody development.
NK Cell Therapy Development
Source material for NK cell-based immunotherapy approaches.
Receptor Profiling
Characterize activating and inhibitory receptor expression patterns.
| Species | Cynomolgusmolgus Monkey (Macaca fascicularis) |
|---|---|
| Cell Type | NK Cells (CD56+/CD16+) |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | PBMC isolation by Ficoll density gradient, followed by immunomagnetic negative selection (NK cell enrichment kit) |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Chinese Origin ★ MOST COMMON
mainland
Chinese-origin cynomolgus are no longer exported by China. Cynomolgus macaques are not native to China and were historically imported from Cambodia, Vietnam, Laos, and Indonesia. Current samples are sourced from US breeding colonies established from China-line imports.
Mauritian Origin ★ DEFINED GENETICS
island
Mauritian cynomolgus have limited MHC diversity due to founder effects, making them valuable for immunology studies requiring genetic consistency.
Cambodian Origin ★ SOUTHEAST ASIAN
mainland
Cambodian-origin animals offer a Southeast Asian source with genetics that have not been mixed with other country lineages in the same way seen in some China-line populations.
Vietnamese Origin ★ SOUTHEAST ASIAN
mainland
Vietnamese cynomolgus provide another Southeast Asian option for programs that require distinct lineage sourcing or supply chain diversification.
Indonesian Origin ★ ISLAND ORIGIN
island
Indonesian-origin cynomolgus were among the earliest origins used in preclinical research and represented one of the largest research populations before China entered the market.
Philippine Origin ★ ISLAND ORIGIN
island
Philippine-origin cynomolgus were one of the first origins used broadly in research, with a long history as a trusted and widely used model.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Typical purity is >80% CD56+/CD16+ NK cells as assessed by flow cytometry. NK cells are enriched by immunomagnetic negative selection to preserve surface receptor integrity. Purity and viability data are included on the Certificate of Analysis.
Many anti-human NK markers cross-react with cynomolgusmolgus and rhesus monkey NK cells, including CD56, CD16, NKG2D, and NKp46. Cross-reactivity varies by antibody clone. We can provide validated clone recommendations for your species.
Thaw rapidly in a 37°C water bath until a small ice crystal remains. Transfer dropwise into pre-warmed NK cell medium with IL-2. Centrifuge at 300g for 10 minutes, resuspend in fresh medium. Allow overnight resting with low-dose IL-2 (100 U/mL) before cytotoxicity or ADCC assays.
Post-thaw viability is typically ≥70% for cryopreserved NK cells. NK cell function (cytotoxicity, ADCC) recovers optimally after overnight IL-2 stimulation. Fresh cells are shipped with ≥90% viability.
Yes. We support donor reservation and repeat-donor programs. Donor demographics and health records are available. NK cell donor variability in cytotoxicity is normal; we can pre-screen donors for high cytotoxic activity on request.
