Cynomolgus Monkey Polymorphonuclear leukocytes (PMNs)
Cynomolgus Monkey primary cells (Macaca fascicularis) isolated using validated methods. Available fresh or cryopreserved with viability guarantee. Contact us for cell-specific specifications.
The Cynomolgus Monkey
Macaca fascicularis
The cynomolgus macaque is the most widely used non-human primate in biomedical research, valued for its close physiological and immunological similarity to humans.
With over 60% genetic homology for key immune receptors, cynomolgus monkeys provide highly translatable data for drug development, making them ideal for immunology, infectious disease, and toxicology studies.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Geographic Origins
Common Research Applications
Cynomolgus Monkey Polymorphonuclear leukocytes (PMNs) is research-grade cynomolgus macaque polymorphonuclear leukocytes (PMNs) intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information and handling details to support consistency across studies.
Common applications
- Preclinical and translational research
- Assay development and validation
- Biomarker studies
- Method development
Collection, processing & handling
Samples are collected and processed using standardized protocols appropriate to the sample type. Packaging and shipping are configured for temperature-controlled logistics to preserve sample integrity for your specific workflow requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Ordering notes
For specific donor criteria, source, volumes, or custom handling requirements, request a quote and include your study specifications. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Immunotoxicology Studies
Assess potential immunotoxic effects of drug candidates on immune cell populations and function.
Vaccine Development
Evaluate immunogenicity and T cell responses to vaccine antigens in preclinical models.
CAR-T & Cell Therapy Research
Source material for developing and testing chimeric antigen receptor constructs and adoptive cell therapies.
Cytokine Release Assays
Screen biologics for potential cytokine release syndrome risk using species-relevant immune cells.
Flow Cytometry & Immunophenotyping
Characterize immune cell subset distributions and surface marker expression profiles.
ADCC & CDC Assays
Evaluate antibody-dependent and complement-dependent cytotoxicity of therapeutic antibodies.
| Species | Cynomolgus Monkey (Macaca fascicularis) |
|---|---|
| Cell Type | Polymorphonuclear Leukocytes (PMNs) |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | Density gradient separation (dextran sedimentation + Ficoll); PMN fraction collected from below the PBMC layer |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Chinese Origin ★ MOST COMMON
mainland
Chinese-origin cynomolgus are no longer exported by China. Cynomolgus macaques are not native to China and were historically imported from Cambodia, Vietnam, Laos, and Indonesia. Current samples are sourced from US breeding colonies established from China-line imports.
Mauritian Origin ★ DEFINED GENETICS
island
Mauritian cynomolgus have limited MHC diversity due to founder effects, making them valuable for immunology studies requiring genetic consistency.
Cambodian Origin ★ SOUTHEAST ASIAN
mainland
Cambodian-origin animals offer a Southeast Asian source with genetics that have not been mixed with other country lineages in the same way seen in some China-line populations.
Vietnamese Origin ★ SOUTHEAST ASIAN
mainland
Vietnamese cynomolgus provide another Southeast Asian option for programs that require distinct lineage sourcing or supply chain diversification.
Indonesian Origin ★ ISLAND ORIGIN
island
Indonesian-origin cynomolgus were among the earliest origins used in preclinical research and represented one of the largest research populations before China entered the market.
Philippine Origin ★ ISLAND ORIGIN
island
Philippine-origin cynomolgus were one of the first origins used broadly in research, with a long history as a trusted and widely used model.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Typical purity is >90% CD3+ T cells as assessed by flow cytometry. Sub-populations (CD4+, CD8+) are further enriched by immunomagnetic positive selection, with purity typically >85-95% depending on the specific marker and species. A Certificate of Analysis with purity data is included with each lot.
Many anti-human CD antibodies cross-react with cynomolgus and rhesus monkey T cell markers, including CD3, CD4, CD8, and CD28. Cross-reactivity varies by clone and species. We can provide guidance on validated antibody clones for your species of interest.
Thaw rapidly in a 37°C water bath until a small ice crystal remains. Transfer dropwise into pre-warmed complete culture medium. Centrifuge at 300g for 10 minutes, aspirate supernatant, and resuspend in fresh medium. Allow 2-4 hours of rest in culture before functional assays for optimal viability and activation response.
Post-thaw viability is typically ≥70% for cryopreserved T cells. Viability is assessed by trypan blue exclusion or flow cytometry (7-AAD/Annexin V). Fresh cells are shipped with viability ≥90%. Recovery and function can be improved with overnight resting before stimulation.
Yes. We support donor reservation, repeat-donor programs, and matched cell sets from the same donor. Donor demographics (age, sex, weight, health status) are available. Contact us with your requirements for custom donor matching.