Cynomolgus Monkey S9 Fraction
Cynomolgus Monkey primary cells (Macaca fascicularis) isolated using validated methods. Available fresh or cryopreserved with viability guarantee. Contact us for cell-specific specifications.
The Cynomolgus Monkey
Macaca fascicularis
The cynomolgus macaque is the most widely used non-human primate in biomedical research, valued for its close physiological and immunological similarity to humans.
With over 60% genetic homology for key immune receptors, cynomolgus monkeys provide highly translatable data for drug development, making them ideal for immunology, infectious disease, and toxicology studies.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Geographic Origins
Common Research Applications
Cynomolgus Monkey S9 Fraction is research-grade cynomolgus macaque S9 liver fraction intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information and handling details to support consistency across studies.
Common applications
- Preclinical and translational research
- Assay development and validation
- Biomarker studies
- Method development
Collection, processing & handling
Samples are collected and processed using standardized protocols appropriate to the sample type. Packaging and shipping are configured for temperature-controlled logistics to preserve sample integrity for your specific workflow requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Ordering notes
For specific donor criteria, source, volumes, or custom handling requirements, request a quote and include your study specifications. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Immunotoxicology Studies
Assess potential immunotoxic effects of drug candidates on immune cell populations and function.
Vaccine Development
Evaluate immunogenicity and T cell responses to vaccine antigens in preclinical models.
CAR-T & Cell Therapy Research
Source material for developing and testing chimeric antigen receptor constructs and adoptive cell therapies.
Cytokine Release Assays
Screen biologics for potential cytokine release syndrome risk using species-relevant immune cells.
Flow Cytometry & Immunophenotyping
Characterize immune cell subset distributions and surface marker expression profiles.
ADCC & CDC Assays
Evaluate antibody-dependent and complement-dependent cytotoxicity of therapeutic antibodies.
| Species | Cynomolgus Monkey (Macaca fascicularis) |
|---|---|
| Product Type | S9 Fraction (Post-mitochondrial Supernatant) |
| Source Tissue | Liver |
| Preparation Method | Liver homogenate centrifuged at 9,000g for 20 min; supernatant (S9) collected |
| Protein Concentration | Typically 10-30 mg/mL total protein |
| Contains | Microsomes + cytosol; both Phase I and Phase II metabolic enzymes |
| Format | Frozen aliquots |
| Storage Buffer | 50 mM Tris-HCl (pH 7.4), 150 mM KCl, 1 mM EDTA |
| Storage Temperature | '-80°C; avoid repeated freeze-thaw cycles |
| Shipping | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Protein concentration measured; enzyme activity data available on request |
| Documentation | Certificate of Analysis with lot-specific data |
| Quality Control | Ames test compatibility verified when applicable |
Geographic Origins Guide
Select the optimal origin for your research requirements
Chinese Origin ★ MOST COMMON
mainland
Chinese-origin cynomolgus are no longer exported by China. Cynomolgus macaques are not native to China and were historically imported from Cambodia, Vietnam, Laos, and Indonesia. Current samples are sourced from US breeding colonies established from China-line imports.
Mauritian Origin ★ DEFINED GENETICS
island
Mauritian cynomolgus have limited MHC diversity due to founder effects, making them valuable for immunology studies requiring genetic consistency.
Cambodian Origin ★ SOUTHEAST ASIAN
mainland
Cambodian-origin animals offer a Southeast Asian source with genetics that have not been mixed with other country lineages in the same way seen in some China-line populations.
Vietnamese Origin ★ SOUTHEAST ASIAN
mainland
Vietnamese cynomolgus provide another Southeast Asian option for programs that require distinct lineage sourcing or supply chain diversification.
Indonesian Origin ★ ISLAND ORIGIN
island
Indonesian-origin cynomolgus were among the earliest origins used in preclinical research and represented one of the largest research populations before China entered the market.
Philippine Origin ★ ISLAND ORIGIN
island
Philippine-origin cynomolgus were one of the first origins used broadly in research, with a long history as a trusted and widely used model.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
S9 fraction (post-mitochondrial supernatant) contains both microsomes (Phase I enzymes: CYPs, FMOs) AND cytosol (Phase II enzymes: GSTs, SULTs, NATs). It provides a more complete metabolic profile than microsomes alone. S9 requires cofactor supplementation (NADPH + UDPGA + PAPS + GSH) for full Phase I + Phase II activity.
Store at -80°C. Avoid repeated freeze-thaw cycles; aliquot upon first thaw. Thaw on ice immediately before use. S9 protein concentration is typically 10-30 mg/mL. Dilute to working concentration (typically 1-2 mg/mL) in incubation buffer.
Standard Ames test S9 mix: 10% v/v S9 fraction in cofactor-supplemented phosphate buffer (NADPH, G6P, G6PDH, MgCl2, KCl). S9 activation mimics mammalian hepatic metabolism for the bacterial mutagenicity assay. Follow OECD 471 guidelines for specific concentrations.
Both individual and pooled donor S9 fractions are available. Pooled preparations (3-10 donors) are standard for regulatory studies to represent population-average metabolism.
Each lot includes: total protein concentration, and a Certificate of Analysis. Enzyme activity data for major CYP isoforms is available on request.