Human Peripheral Blood Mononuclear Cells (PBMC)
High-quality Human PBMCs (Homo sapiens) isolated by density gradient centrifugation. Available fresh or cryopreserved with guaranteed viability. Ideal for immunology, vaccine development, and cell-based assays in preclinical research.
Human Overview
Human primary cells and biofluids from ethically consented, IRB-approved donors provide the ultimate translational relevance. ODIN supplies normal and disease-state human samples with full donor demographics and consent documentation.
Quick Facts
Human Peripheral Blood Mononuclear Cells (PBMC) are research-grade human peripheral blood mononuclear cells (PBMCs) intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information including viability data, cell counts, and handling details to support consistency across studies.
PBMCs are isolated mononuclear cells including lymphocytes and monocytes, critical for immunological research requiring viable, functional immune cells.
Available fresh or cryopreserved to match your experimental timeline and viability requirements.
Common applications
- T cell and B cell functional assays
- Flow cytometry and immunophenotyping
- Cytokine secretion and ELISpot assays
- Mixed lymphocyte reactions (MLR)
- CAR-T and cell therapy development
- Vaccine immunogenicity studies
Collection, processing & handling
PBMCs are isolated using density gradient separation with standardized protocols appropriate to the donor species. Fresh PBMCs are shipped in validated containers with temperature monitoring; cryopreserved PBMCs are shipped on dry ice with documented freeze-thaw protocols. Viability testing available upon request.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Also available: matched serum or plasma for comprehensive immune profiling from the same species for comprehensive study design.
Ordering notes
For specific donor criteria, source, volumes, or custom handling requirements, request a quote and include your study specifications. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Immunotoxicology Studies
Assess potential immunotoxic effects of drug candidates on immune cell populations and function.
Vaccine Development
Evaluate immunogenicity and T cell responses to vaccine antigens in preclinical models.
CAR-T & Cell Therapy Research
Source material for developing and testing chimeric antigen receptor constructs and adoptive cell therapies.
Cytokine Release Assays
Screen biologics for potential cytokine release syndrome risk using species-relevant immune cells.
Flow Cytometry & Immunophenotyping
Characterize immune cell subset distributions and surface marker expression profiles.
ADCC & CDC Assays
Evaluate antibody-dependent and complement-dependent cytotoxicity of therapeutic antibodies.
| Species | Human (Homo sapiens) |
|---|---|
| Cell Type | Peripheral Blood Mononuclear Cells (PBMCs) |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | Density gradient centrifugation (Ficoll) |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Human PBMCs contain lymphocytes (T cells, B cells, NK cells) and monocytes. Typical composition includes 60-80% T cells (CD3+), 5-15% B cells (CD20+), 5-15% NK cells (CD56+), and 5-20% monocytes (CD14+). Exact percentages vary by individual donor and species.
Many anti-human antibodies cross-react with human cells due to epitope conservation. We recommend consulting cross-reactivity databases and validating antibodies empirically. Common cross-reactive markers include CD3, CD4, CD8, CD14, CD20, and CD56.
Thaw vials rapidly in a 37°C water bath until a small ice crystal remains. Dilute slowly into pre-warmed complete medium, centrifuge gently (300 × g, 10 min), and resuspend in fresh medium. Rest cells for 1-2 hours before functional assays.
Post-thaw viability is guaranteed ≥70%. Actual viability typically ranges 75-90% when following recommended thawing protocols. Functional recovery may require 2-4 hours of resting.
Yes, we can provide cells from specific donors for longitudinal studies or matched sample requests. Contact us with your requirements for donor availability and additional donor information.