Rat Bone Marrow MNCs
Rat primary cells (Rattus norvegicus) isolated using validated methods. Available fresh or cryopreserved with viability guarantee. Contact us for cell-specific specifications.
Sprague Dawley Rat Overview
The Sprague Dawley is an outbred albino rat and the most widely used rat strain in pharmacology, toxicology, and safety testing. Its calm temperament, rapid growth, and extensive historical control data make it a regulatory mainstay.
Quick Facts
Rat Bone Marrow MNCs is research-grade rat bone marrow intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information and handling details to support consistency across studies.
Bone marrow provides access to hematopoietic stem cells and progenitor populations essential for hematology, oncology, and regenerative medicine research.
Common applications
- Hematopoietic stem cell (HSC) research
- Colony-forming unit (CFU) assays
- Bone marrow transplant modeling
- Hematological malignancy studies
- Myeloid and lymphoid differentiation research
Collection, processing & handling
Bone marrow is collected via aspiration with documented protocols. Available as whole aspirate, mononuclear cell preparations, or processed fractions. Shipped fresh or cryopreserved depending on downstream application requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Also available: matched peripheral blood for comparative hematopoiesis studies from the same species for comprehensive study design.
Ordering notes
For specific donor criteria, strain, volumes, or custom handling requirements, request a quote and include your study specifications. Available strains include Sprague Dawley and Wistar. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Immunotoxicology Studies
Assess potential immunotoxic effects of drug candidates on immune cell populations and function.
Vaccine Development
Evaluate immunogenicity and T cell responses to vaccine antigens in preclinical models.
CAR-T & Cell Therapy Research
Source material for developing and testing chimeric antigen receptor constructs and adoptive cell therapies.
Cytokine Release Assays
Screen biologics for potential cytokine release syndrome risk using species-relevant immune cells.
Flow Cytometry & Immunophenotyping
Characterize immune cell subset distributions and surface marker expression profiles.
ADCC & CDC Assays
Evaluate antibody-dependent and complement-dependent cytotoxicity of therapeutic antibodies.
| Species | Rat (Rattus norvegicus) |
|---|---|
| Cell Type | Bone Marrow Mononuclear Cells (BM-MNCs) |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | Ficoll density gradient centrifugation of bone marrow aspirate from iliac crest |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Bone marrow MNCs contain a higher proportion of hematopoietic progenitors (CD34+ cells), mesenchymal stromal cells, and early immune cell precursors compared to PBMCs. The myeloid-to-lymphoid ratio is also different, with more myeloid precursors in BM-MNCs.
Bone marrow is aspirated from the iliac crest under anesthesia. The aspirate is processed by Ficoll density gradient centrifugation to isolate mononuclear cells. The procedure is similar to PBMC isolation but starts from bone marrow aspirate rather than peripheral blood.
Thaw rapidly at 37°C, dilute dropwise into pre-warmed medium. Add DNase I (10 µg/mL) to reduce clumping. Centrifuge at 300g for 10 minutes and resuspend in fresh medium. Rest 2-4 hours before plating.
Post-thaw viability is typically ≥70% for BM-MNCs. CD34+ progenitor content and colony-forming capacity are maintained post-thaw. Fresh cells are recommended for engraftment or long-term culture studies.
Yes. Donor reservation and matched sample sets (BM-MNCs + PBMCs + serum) are available. Donor demographics and health records are provided with each lot.