Rhesus Monkey CD14+ Monocytes
Purified Rhesus Monkey CD14+ monocytes (Macaca mulatta) for macrophage differentiation, dendritic cell generation, and inflammation research. High purity isolation by immunomagnetic selection.
The Rhesus Monkey
Macaca mulatta
The rhesus macaque is one of the most studied primate species, fundamental to biomedical research for over a century.
With ~93% genetic homology to humans and extensive immunological characterization, rhesus monkeys are essential for vaccine development, HIV/AIDS research, and neuroscience studies.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Geographic Origins
Common Research Applications
Rhesus Monkey CD14+ Monocytes are research-grade rhesus macaque primary cells intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information including viability and purity data.
CD14+ monocytes are innate immune cells that differentiate into macrophages and dendritic cells, supporting inflammation and phagocytosis research.
Common applications
- Monocyte-to-macrophage differentiation
- Dendritic cell generation
- Inflammation research
- Phagocytosis assays
- Innate immunity studies
Collection, processing & handling
Samples are collected and processed using standardized protocols appropriate to the sample type. Packaging and shipping are configured for temperature-controlled logistics to preserve sample integrity for your specific workflow requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Ordering notes
For specific donor criteria, source, volumes, or custom handling requirements, request a quote and include your study specifications. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Macrophage Differentiation
Generate monocyte-derived macrophages for functional studies.
Dendritic Cell Generation
Differentiate monocytes into dendritic cells for antigen presentation studies.
Inflammation Research
Study monocyte activation and inflammatory cytokine production.
Phagocytosis Assays
Evaluate phagocytic function and clearance mechanisms.
| Species | Rhesus Monkey (Macaca mulatta) |
|---|---|
| Cell Type | CD14+ Monocytes |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | PBMC isolation by Ficoll density gradient, followed by immunomagnetic positive selection (anti-CD14) |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Chinese Origin ★ MOST COMMON
asia
Chinese-origin rhesus are commonly used in toxicology and translational safety programs, supported by established breeding colonies and robust historical datasets.
Indian Origin
india lineage
Indian-origin rhesus represent a historically important research lineage, maintained through domestic breeding programs with strong legacy use in biomedical studies.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Typical purity is >90% CD14+ monocytes as assessed by flow cytometry. Monocytes are isolated by immunomagnetic positive selection from PBMCs. Purity and viability data are included on the Certificate of Analysis.
Yes. CD14+ monocytes can be differentiated into macrophages using M-CSF (5-7 days) or into monocyte-derived dendritic cells (moDCs) using GM-CSF + IL-4 (5-7 days). Differentiation protocols are available upon request.
Thaw rapidly at 37°C, transfer dropwise into pre-warmed RPMI + 10% FBS. Centrifuge at 300g for 10 minutes. Resuspend in fresh medium and plate immediately. Monocytes are adherent; allow 2-4 hours for attachment before washing non-adherent cells.
Post-thaw viability is typically ≥70%. Monocyte adhesion and phagocytic function recover well after thawing. Fresh cells are shipped with ≥90% viability. For differentiation assays, fresh monocytes are recommended.
Yes. Donor reservation and repeat-donor programs are available. Donor demographics, health status, and HLA typing (if available) can be provided on request.