Rhesus Monkey NK Cells
Purified Rhesus Monkey NK cells (Macaca mulatta) for cytotoxicity assays, ADCC studies, and NK cell therapy development. Isolated by immunomagnetic selection with high purity.
The Rhesus Monkey
Macaca mulatta
The rhesus macaque is one of the most studied primate species, fundamental to biomedical research for over a century.
With ~93% genetic homology to humans and extensive immunological characterization, rhesus monkeys are essential for vaccine development, HIV/AIDS research, and neuroscience studies.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Geographic Origins
Common Research Applications
Rhesus Monkey NK Cells are research-grade rhesus macaque primary cells intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information including viability and purity data.
Natural killer (NK) cells are innate immune effectors critical for cancer immunotherapy research and viral immunity studies.
Common applications
- Cancer immunotherapy development
- NK cell-mediated cytotoxicity
- Viral immunity research
- Antibody-dependent cellular cytotoxicity (ADCC)
- Innate immune profiling
Collection, processing & handling
Samples are collected and processed using standardized protocols appropriate to the sample type. Packaging and shipping are configured for temperature-controlled logistics to preserve sample integrity for your specific workflow requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Ordering notes
For specific donor criteria, source, volumes, or custom handling requirements, request a quote and include your study specifications. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Cytotoxicity Assays
Evaluate NK cell-mediated killing of target cells.
ADCC Studies
Assess antibody-dependent cellular cytotoxicity for therapeutic antibody development.
NK Cell Therapy Development
Source material for NK cell-based immunotherapy approaches.
Receptor Profiling
Characterize activating and inhibitory receptor expression patterns.
| Species | Rhesus Monkey (Macaca mulatta) |
|---|---|
| Cell Type | NK Cells (CD56+/CD16+) |
| Cell Count | ≥10 × 10⁶ viable cells per vial (standard) |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | PBMC isolation by Ficoll density gradient, followed by immunomagnetic negative selection (NK cell enrichment kit) |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Chinese Origin ★ MOST COMMON
asia
Chinese-origin rhesus are commonly used in toxicology and translational safety programs, supported by established breeding colonies and robust historical datasets.
Indian Origin
india lineage
Indian-origin rhesus represent a historically important research lineage, maintained through domestic breeding programs with strong legacy use in biomedical studies.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Typical purity is >80% CD56+/CD16+ NK cells as assessed by flow cytometry. NK cells are enriched by immunomagnetic negative selection to preserve surface receptor integrity. Purity and viability data are included on the Certificate of Analysis.
Many anti-human NK markers cross-react with cynomolgus and rhesus monkey NK cells, including CD56, CD16, NKG2D, and NKp46. Cross-reactivity varies by antibody clone. We can provide validated clone recommendations for your species.
Thaw rapidly in a 37°C water bath until a small ice crystal remains. Transfer dropwise into pre-warmed NK cell medium with IL-2. Centrifuge at 300g for 10 minutes, resuspend in fresh medium. Allow overnight resting with low-dose IL-2 (100 U/mL) before cytotoxicity or ADCC assays.
Post-thaw viability is typically ≥70% for cryopreserved NK cells. NK cell function (cytotoxicity, ADCC) recovers optimally after overnight IL-2 stimulation. Fresh cells are shipped with ≥90% viability.
Yes. We support donor reservation and repeat-donor programs. Donor demographics and health records are available. NK cell donor variability in cytotoxicity is normal; we can pre-screen donors for high cytotoxic activity on request.