Rhesus Monkey Splenocytes
Rhesus Monkey primary cells (Macaca mulatta) isolated using validated methods. Available fresh or cryopreserved with viability guarantee. Contact us for cell-specific specifications.
The Rhesus Monkey
Macaca mulatta
The rhesus macaque is one of the most studied primate species, fundamental to biomedical research for over a century.
With ~93% genetic homology to humans and extensive immunological characterization, rhesus monkeys are essential for vaccine development, HIV/AIDS research, and neuroscience studies.
Human Similarity
~93% genetic homology with extensive immune receptor cross-reactivity
Global Availability
Multiple geographic origins with established breeding colonies
Regulatory Accepted
FDA & EMA preferred species for biologics safety assessment
Geographic Origins
Common Research Applications
Rhesus Monkey Splenocytes are primary immune cells isolated from rhesus macaque (Macaca mulatta) spleen tissue. The rhesus macaque is the most widely used NHP in biomedical research, with extensive immunological characterization and reagent cross-reactivity with human markers.
Common Applications
- Vaccine immunogenicity and efficacy evaluation
- ELISpot, FluoroSpot, and cytokine profiling
- SIV/SHIV and infectious disease research
- Immune reconstitution and transplant tolerance studies
Product Details
Splenocytes are prepared by mechanical dissociation and cryopreserved. Each lot ships with a Certificate of Analysis documenting viability, cell count, and donor information including origin. Available in 10M, 25M, and 50M cell counts.
Immunotoxicology Studies
Assess potential immunotoxic effects of drug candidates on immune cell populations and function.
Vaccine Development
Evaluate immunogenicity and T cell responses to vaccine antigens in preclinical models.
CAR-T & Cell Therapy Research
Source material for developing and testing chimeric antigen receptor constructs and adoptive cell therapies.
Cytokine Release Assays
Screen biologics for potential cytokine release syndrome risk using species-relevant immune cells.
Flow Cytometry & Immunophenotyping
Characterize immune cell subset distributions and surface marker expression profiles.
ADCC & CDC Assays
Evaluate antibody-dependent and complement-dependent cytotoxicity of therapeutic antibodies.
| Species | Rhesus Monkey (Macaca mulatta) |
|---|---|
| Cell Type | Splenocytes (Total Spleen Mononuclear Cells) |
| Cell Count | Variable; typically 50-500 × 10⁶ cells per spleen depending on species |
| Viability (Fresh) | ≥90% |
| Viability (Post-Thaw) | ≥70% |
| Viability Method | Trypan blue exclusion or flow cytometry |
| Format | Fresh or cryopreserved |
| Cryopreservation Medium | Serum-containing medium with 10% DMSO |
| Isolation Method | Mechanical dissociation of spleen tissue through cell strainer, followed by red blood cell lysis |
| Storage (Cryopreserved) | Liquid nitrogen vapor phase |
| Shipping (Fresh) | Ambient temperature, overnight delivery |
| Shipping (Cryopreserved) | Dry ice |
| Donor Information | Age, sex, and health status available |
| Testing | Pathogen-free per supplier SOPs |
Geographic Origins Guide
Select the optimal origin for your research requirements
Chinese Origin ★ MOST COMMON
asia
Chinese-origin rhesus are commonly used in toxicology and translational safety programs, supported by established breeding colonies and robust historical datasets.
Indian Origin
india lineage
Indian-origin rhesus represent a historically important research lineage, maintained through domestic breeding programs with strong legacy use in biomedical studies.
Need help selecting an origin?
Our scientific team can advise on the optimal origin for your study design and regulatory requirements.
Splenocytes contain a mixed immune cell population including T cells (40-60%), B cells (30-50%), NK cells (5-10%), monocytes/macrophages, and dendritic cells. The exact composition varies by species and individual donor. Flow cytometry immunophenotyping data is available on request.
Splenocytes are isolated by gentle mechanical dissociation of the spleen through a cell strainer, followed by red blood cell lysis (ACK buffer). The resulting single-cell suspension is washed and resuspended in culture medium or cryopreservation medium.
Thaw rapidly at 37°C, dilute dropwise into pre-warmed RPMI + 10% FBS. Centrifuge at 300g for 10 minutes. Resuspend in fresh medium and rest overnight before functional assays for optimal recovery.
Post-thaw viability is typically ≥70%. Splenocyte function (proliferation, cytokine production) recovers well after overnight resting. Fresh splenocytes are shipped with ≥90% viability.
Yes. Donor demographics (age, sex, weight, health status) are available. Matched sets (splenocytes + PBMCs + serum from the same donor) can be arranged.