Wistar Rat S9 Fraction
Wistar Rat (Rattus norvegicus) cell product collected under controlled conditions. Processed promptly for optimal quality. Contact us for specifications and availability.
Wistar Rat S9 Fraction Overview
Wistar Rat S9 Fraction is research-grade rat S9 fraction intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information and handling details to support consistency across studies.
Common applications
- Preclinical and translational research
- Assay development and validation
- Biomarker studies
- Method development
Collection, processing & handling
Samples are collected and processed using standardized protocols appropriate to the sample type. Packaging and shipping are configured for temperature-controlled logistics to preserve sample integrity for your specific workflow requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Ordering notes
For specific donor criteria, strain, volumes, or custom handling requirements, request a quote and include your study specifications. Available strains include Sprague Dawley and Wistar. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Wistar Rat Overview
The Wistar is the original laboratory rat strain and progenitor of many modern lines. This outbred albino rat is a versatile, well-characterized model used broadly across physiology, pharmacology, and behavioral research.
Quick Facts
Metabolic Activation Studies
Activate promutagens for genotoxicity testing (Ames test, micronucleus).
Drug Metabolism
Combined Phase I and Phase II metabolism studies.
Metabolite Generation
Generate both oxidative and conjugative metabolites.
In Vitro Toxicity
Assess toxicity of parent compounds and metabolites.
Metabolic Stability
Determine combined Phase I/II metabolic stability.
Reactive Metabolite Studies
Investigate reactive metabolite formation and protein binding.
| Species | Wistar Rat (Rattus norvegicus) |
|---|---|
| Sample Type | S9 Fraction (9000g supernatant) |
| Induction | Aroclor-1254 or phenobarbital/beta-naphthoflavone |
| Protein Content | Characterized per lot |
| Enzyme Activities | CYP and Phase II enzymes present |
| Storage | Frozen at -80C |
S9 contains both microsomal (ER) and cytosolic enzymes, enabling Phase I and Phase II metabolism. Microsomes contain only ER enzymes.
Yes, our standard S9 is from Aroclor-1254 induced rats. Uninduced and other induction protocols available.
NADPH for CYP reactions. UDPGA, PAPS, GSH, and acetyl-CoA for Phase II reactions.
Standard Ames test uses S9 at 5-10% (v/v) final concentration.