Sprague Dawley Rat Splenocytes
Sprague Dawley Rat (Rattus norvegicus) cell product collected under controlled conditions. Processed promptly for optimal quality. Contact us for specifications and availability.
Sprague Dawley Rat Splenocytes Overview
Sprague Dawley Rat Splenocytes is research-grade rat splenocytes intended for preclinical and translational research. Each order includes a Certificate of Analysis (COA) with lot-specific information and handling details to support consistency across studies.
Splenocytes provide a rich source of lymphocytes and antigen-presenting cells directly from lymphoid tissue, supporting studies of adaptive immunity and antigen presentation.
Common applications
- Lymphocyte proliferation assays
- Antigen-specific immune response studies
- B cell and antibody research
- Immune cell co-culture experiments
- Vaccine and immunotherapy development
Collection, processing & handling
Samples are collected and processed using standardized protocols appropriate to the sample type. Packaging and shipping are configured for temperature-controlled logistics to preserve sample integrity for your specific workflow requirements.
Quality control & documentation
QC testing options available on request, including material-appropriate analytical checks and extended documentation. Standard documentation includes collection/processing metadata, lot identifiers, and chain-of-custody records. Custom QC panels can be configured for GLP or regulatory-facing studies.
Also available: matched spleen tissue for histological correlation from the same species for comprehensive study design.
Ordering notes
For specific donor criteria, strain, volumes, or custom handling requirements, request a quote and include your study specifications. Available strains include Sprague Dawley and Wistar. We routinely support longitudinal programs, matched sample sets, and custom collection protocols.
Sprague Dawley Rat Overview
The Sprague Dawley is an outbred albino rat and the most widely used rat strain in pharmacology, toxicology, and safety testing. Its calm temperament, rapid growth, and extensive historical control data make it a regulatory mainstay.
Quick Facts
Immunophenotyping
Characterize splenic immune cell populations by flow cytometry.
Mixed Lymphocyte Reactions
Assess allogeneic immune responses for transplant and immunology research.
ELISPOT Assays
Detect antigen-specific T and B cell responses at single-cell resolution.
Cytokine Studies
Measure cytokine production from splenic immune cells.
Vaccine Research
Evaluate immune responses to vaccine candidates.
Antibody Generation
Source of B cells for hybridoma production and antibody development.
| Species | Sprague Dawley Rat (Rattus norvegicus) |
|---|---|
| Cell Type | Splenocytes (mixed population) |
| Source | Fresh spleen tissue |
| Isolation Method | Mechanical dissociation and RBC lysis |
| Viability | >90% (typical) |
| Storage | Fresh or cryopreserved |
Splenocytes contain T cells, B cells, NK cells, monocytes, and dendritic cells in species-specific proportions.
Yes, we can isolate specific cell types from splenocytes including T cells, B cells, or dendritic cells.
Typical yield is 100-200 million cells per rat spleen.
Thaw rapidly at 37C and dilute immediately in pre-warmed medium containing DNase to prevent clumping.